Of these 74 predicted targets of miR-509, 12 genes previously demonstrated in the literature to be either involved in leukemia and oncogenesis (ERLIN2, FLI1, FOXP1, MAML1, RAC1, YWHAB and YWHAG), or predicted as miR-509 targets by both TargetScan6.2 and miRDB (PGRMC1, RAB5C, RAC1, TFDP2, UHMK1, USP9X) were selected for initial qRT-PCR analysis. This evidence concerns the gene PGRMC1 and leukemia.