The mean surface density of Podoplanin on HEK293T cells, human dermal microvascular endothelial (HMEC-1) cells, and squamous cell carcinoma (FaDu) cells was determined by comparing Podoplanin antibody-stained cells to calibration beads with known antigen binding capacities (Table 1). This evidence concerns the gene PDPN and squamous cell carcinoma.