In another experiment we incubated neuroblastoma cells with pharmacological inhibitors specific for Rac1 (NSC23766; 25 μM) or Cdc42 (ML141; 10 μM) one hour prior to metformin (10 mM) treatment, and cell viability was accessed after 4 days (SKN-BE(2) cells) and 6 days (SH-SY5Y cells) of treatments by trypan blue. Here, RAC1 is linked to neuroblastoma.