We further demonstrated the involvement of EMT in glioma invasion and tested by western blot analysis the effect of shOLIG2 on the expression of central regulators of EMT during neural crest cell migration and cancer: TWIST, SNAI2 (SLUG) and SOX9. Our analysis revealed (Figure 6G) that 0923 shOLIG2 GSCs exhibit reduced levels of SNAI2 and TWIST and elevated SOX9 levels. This evidence concerns the gene TWIST1 and central nervous system cancer.