Figure 5 shows significant expression of OLIG2 in cell nuclei and reduced expression of PARD3 in plasma membrane in the AOI compared to the tumor core. EFNB3 was enriched in the plasma membrane and cytoplasm of host cells with an astrocytic appearance that reside within the AOI. The staining for OLIG2, PARD3 and EFNB3 showed marked heterogeneity between patients in terms of both intensity and distribution, ranging from a few weakly positive cells to apparent overexpression. The gene discussed is EFNB3; the disease is neoplasm.