ACTB and cervical cancer: However, even though it has been widely accepted that one of the best ways to normalize the qPCR data is to use at least 2 to 3 reference genes, several studies of cervical cancer continue to use the most well-known reference genes such as GAPDH[23], [45], ACTB[30], EEF1A1 [46] and RNU6[15], [24]–[27], [46]–[51], as a single reference gene and without mentioning whether this stage has been performed accurately.