The average scores of IHC for Msi1 were 3.67±2.72 in NC, 4.27±2.39 in CIS, 7.10±2.90 in ICC (Fig. 1C, NC vs CIS, P>0.05; NC vs ICC, P<0.001; CIS vs ICC, P<0.001). This evidence concerns the gene MSI1 and in situ carcinoma.