To support the hypothesis that Msi1 could possibly down-regulate the expression of p21, p27 and p53 in cervical carcinoma tissues, the expression levels of Msi1 and p21, p27, p53 in 13 human cervical cancer samples were examined using immunohistochemistry in 4-μM consecutive tissue sections (Fig. 6F). This evidence concerns the gene CDKN1B and cervical carcinoma.