At last, in order to identify the effects on the proliferation of cervical carcinoma cells of the functional p21, p27 and p53 exerted by Msi1, rescue treatments were performed by transfecting Msi1 sponge vectors containing 2×binding sites fragments of p21, p27 and p53 in both Msi1 overexpressing HeLa (Fig.7A) and SiHa cells (Fig.7B). Here, CDKN1B is linked to cervical carcinoma.