MSI1 and intrahepatic cholangiocarcinoma: Msi1 positive staining localized in nucleus and/or cytoplasm (Fig. 1A) was found in 30% (9 of 30) of the NC samples, in 43.3% (13 of 30) of the CIS samples and in 81.4% (48 of 59) of the ICC samples (Fig. 1B, NC vs CIS, P>0.05; NC vs ICC, P<0.001; CIS vs ICC, P<0.05).