However, transient transfection of a plasmid with high ANXA2 expression restored the migration and invasive ability of cells with low ANXA2 expression and this effect was enhanced by extrinsic HE4 active protein supplementation (P <0.05; Figure 4D, E, F), which further validated that HE4 and ANXA2 binding promotes ovarian cancer cell invasion and migration. This evidence concerns the gene ANXA2 and ovarian carcinoma.