The major findings of the present study are: (1) Ivabradine treatment significantly inhibits the expression and activity of MMP-2 in diabetic mice, (2) Ivabradine down-regulates the phosphorylation of Caspase 3, BAX, but up-regulats the phosphorylation of NF-kB in mice with diabetes. This evidence concerns the gene NFKB1 and diabetes mellitus.