Here, we firstly reported that LDH-A inhibition induced autophagy in human non-small cell lung cancer A549 cells, as evidenced by appearance of autophagic vacuoles and LC-3 degradation, our data also showed that the Akt-mTOR signaling pathway was inhibited significantly after oxamate treatment, and might involve in the regulation of the metabolism and autophagy induced by LDH-A inhibition. The gene discussed is MAP1LC3A; the disease is non-small cell lung carcinoma.