In conclusion, we find that NSCLC cells exhibit different responses to LDH-A inhibition in our study, and provide novel insights into the signaling pathways shifting cancer cells towards apoptosis or autophagy, as well as different cell cycle arrests, which are helpful for searching biomarkers to monitor the efficacy of glycolysis inhibitors and contribute to more favorable outcomes in the future clinical trials. Here, LDHA is linked to non-small cell lung carcinoma.