Sections of brain and liver, stained by H&E and immunostained for viral antigen and the microglial marker Iba1, confirmed successful inoculation in two of the RSA59 infected group by presence of encephalitis and hepatic inflammation in RSA59-infected mice, and no inflammatory lesions in control mice (Figure S1), similar to prior studies [14]. This evidence concerns the gene AIF1 and encephalitis.