SLC12A5 and central nervous system cancer: To disclose the effects of glioma co-culture on neuronal KCC2 protein expression, we performed immunoblot experiments, revealing that the level of transporter expression was similar in control and co-cultured neurons (Figure 3d), thus indicating that glioma-induced alteration of neuronal Cl− homeostasis relies on functional KCC2 modulation, rather than on changes in expression.