We provide evidence that glioma-induced KCC2 impairment, observed in co-cultured neurons, is due to intracellular Zn2+ rise.18 Indeed, intracellular Zn2+ chelation by TPEN rapidly reverts co-culture-induced EGABA shift and basal [Zn2+]i is significantly higher in neurons after glioma co-culture. The gene discussed is SLC12A5; the disease is glioma.