Ectopic expression of RIPK3 rescued IZ-induced TNFα in HEY cells (Figure 5c), suggesting that RIPK3 expression may enhance autocrine TNFα production by the ovarian cancer cells as it does in myeloid cells.20 The kinase activity of RIPK3 was critical for this effect, as the expression of catalytically dead RIPK3 (K50A) did not induced the expression of TNFα (Figure 5c). This evidence concerns the gene TNF and ovarian cancer.