The previous study had revealed that UCN-01 could induce Puma-related apoptosis in a p53-independent manner.25 In this study, we proved these results and further provided the evidence that UCN-01 could induce Puma expression in other cancer cells with dysfunctional p53 such as A2780/CP7 and K526.26 Moreover, our data demonstrated that UCN-01 triggered Puma induction in CP-resistant cancer cells (A2780/CP and K526/CP) and confirmed that UCN-01 could function as a chemosensitizer. Here, PRB2 is linked to cancer.