To examine whether similar IRG-related processes might also occur on the microsporidian vacuole, we co-stained IFNγ-treated, E. cuniculi-infected, MEF cells with immunological reagents against individual GKS effector proteins (Irga6, Irgb6 and Irgd) as well against the GMS regulator proteins (Irgm1 and Irgm2) 24 h post infection (Figure 2). The gene discussed is IFNG; the disease is infection.