Congruently, by using flow cytometric analysis with Annexin V/PI staining, we revealed that the spontaneous apoptotic rates of LRIG2 overexpressing U87 and U251 cells were significantly decreased compared to the corresponding control cells (Figure 5A), indicating that LRIG2 overexpression inhibited the spontaneous apoptosis of glioblastoma cells. This evidence concerns the gene ANXA5 and glioblastoma.