With the strict threshold, 6 highly expressed genes were screened in GBM, including AFF2, GNAL, ARPP21, CACNA2D3 and RGS7, in addition to 6 highly expressed genes in OD, including CNTN2, ABCA6, MEGF11, DOCK5, MOXD1 and TRIM67. Finally, by constructing a PPI network of DEGs, it was demonstrated that TP53 and HIST1H3A were the hub nodes in the PPI network of GBM and CNTN2 was the hub node in OD. The gene discussed is AFF2; the disease is osteochondritis dissecans.