Finally, significant main effects of treatment group (F3,90 = 9.78; P < 0.001) and time of intervention (F2,90 = 9.35; P < 0.001) were observed for mRNA expression of the anti‐inflammatory marker IL‐10 (Fig. 3E), with IL‐10 gene expression increasing over time (P < 0.01) and animals in the HF groups having greater mRNA expression than animals in the LF groups (P < 0.05). The gene discussed is IL10; the disease is hydrops fetalis.