The CFS was initially validated using two model antigens (HA1 from influenza A/Texas/1/77 and type II collagen) as positive controls and then applied prospectively for the discovery of new HLA-DRB1*0101 immunodominant epitopes from a recombinant liver-stage antigen of malaria falciparum (LSA-NRC) and HA1 from H5N1 influenza (Viet Nam). This evidence concerns the gene NCOA6 and malaria.