AKT1 and non-small cell lung carcinoma: Thus, in the present study, anti-metastatic mechanism of EEOS was elucidated via cell viability assay, cell adhesion and invasion assays, ELISA for MMP-9, RT-PCR for uPA, uPAR and EGFR, Western blotting for osteopontin (OPN), and CD44, ELISA for VEGF and PI3K/Akt inhibitor LY294002 study in OPN treated NCI-H460 non-small cell lung cancer cells.