To examine the regulation of TM4SF4 expression by methylation in the NSCLC cells tested above, puta­tive CpG islands within the promoter and 5′-untranslated region (5′-UTR) of the TM4SF4 gene were analyzed using the Methprimer program (http://www.urogene.org//methprimer) [25], and two CpG islands were suggested as methylation sites (Figure 1B). Here, TM4SF4 is linked to non-small cell lung carcinoma.