Although we did not investigated further about the induction mechanism or action of IL1beta related to tumorigenicity of TM4SF4-overexpressed lung adenocarcinoma cells in this study, NF-kappaB and PI3K signaling, key components regulating the expression of IGF1 in A549 cells, also regulate the expression of IL1beta as shown in results and investigation about its action in lung cancer cells might show another way of cancer treatment. This evidence concerns the gene TM4SF4 and lung cancer.