SPRY4 and melanoma: Despite the similar expression patterns of SPRY4 and SPRY4-IT1, siRNA-mediated knockdown of SPRY4 (siRNA targeting exon 1) in A375 melanoma cells reduced the expression of SPRY4 but not SPRY4-IT1 (Fig. 1D), demonstrating that transcription of the coding (SPRY4) and noncoding (SPRY4-IT1) genes occurred independently.