The bright field images also indicate that monolayer cells treated with 200 ng/mL of TRAIL show morphological changes characteristic of apoptosis (more rounded and suspended) whereas cells cultured as tumor spheroids were more resistant to TRAIL-mediated apoptosis (Fig. 2C and 2D) with most of the cells viable (spread out and adherent) after TRAIL treatment. The gene discussed is TNFSF10; the disease is neoplasm.