In addition, in five NSCLC cell lines (A549, H1299, H157, SK-MES-1 and H358) that showed decreased miR-486-5p expression relative to that of human normal lung tissue (Figure 4F), Pim-1 protein expressions were all indicated at relatively higher level (Figure 4G and Additional file 3: Figure S2). The gene discussed is PIM1; the disease is non-small cell lung carcinoma.