MiR-486-5p was downregulated in 87.5% (21of 24) tumor tissues compared with the matched normal lung tissues (Figure 4E and Additional file 1: Table S1), whereas Pim-1 protein was upregulated in 87.5% (21 of 24) tumor tissues by Western bolt analysis (Figure 1C). This evidence concerns the gene PIM1 and neoplasm.