As expected, the β-catenin/TCF4 activity and the expression levels of eight classically recognized Wnt/β-catenin target genes were significantly increased in the TBLR1 overexpressing breast cancer cells, but decreased in the TBLR1 silencing cells (Figure 6C and D, Additional file 3: Figure S3). This evidence concerns the gene TBL1XR1 and breast cancer.