Similarly, western blotting and IHC showed that TBLR1 protein was upregulated in breast cancer tissue samples compared to their matched adjacent non-tumor tissues from the same patient (Figure 1B and C); and real-time PCR revealed that TBLR1 mRNA was also significantly upregulated in breast cancer tissues (Additional file 1: Figure S1B). Here, TBL1XR1 is linked to breast cancer.