The A549 cell line is a widely used NSCLC defined by high expression of Mcl-1.26 We first confirmed that native Mcl-1 and VDAC1/3 interact in this cell line using a proximity ligation assay (PLA) to detect and quantify the endogenous protein complexes at the single cell level.28 The Mcl-1/VDAC complexes were labeled using an antibody directed against Mcl-1 and an antibody known to react with both the VDAC1 and 3 isoforms. This evidence concerns the gene VDAC1 and non-small cell lung carcinoma.