To determine if TRAF6 played a more ubiquitous role in MCL-1 stabilization and survival of cancer cell lines, TRAF6 was knocked down with shRNA in HeLa (cervical carcinoma), MCF-7 (breast carcinoma), DLD-1 (colorectal adenocarcinoma) and 293 cells. The gene discussed is TRAF6; the disease is colorectal adenocarcinoma.