Sequencing of DNA isolated directly from a fixed tumor specimen of one individual demonstrated the presence of a de novo second-hit variant in MAP3K6. Screening of an additional 115 unrelated FGC samples, also negative for CDH1 mutations, revealed five individuals with four additional SNVs in MAP3K6 that were also predicted to be pathogenic, as well as an unrelated individual with the SNV identified in the family from Maritime Canada. This evidence concerns the gene MAP3K6 and neoplasm.