CSF1 and osteosarcoma: In an immunocompetent mouse model of osteosarcoma, in which mice were subcutaneously transplanted with the mouse AX osteosarcoma cell line, the M-CSF inhibitor Ki20227 or the AFS98 rat anti-murine M-CSFR mAb dramatically decreased peritumoral and perivascular TAM, suppressed tumor angiogenesis and lymphangiogenesis, disorganized extracellular matrices and concomitantly dramatically suppressed metastasis and improved prognosis (102).