In an immunocompetent mouse model of osteosarcoma, in which mice were subcutaneously transplanted with the mouse AX osteosarcoma cell line, the M-CSF inhibitor Ki20227 or the AFS98 rat anti-murine M-CSFR mAb dramatically decreased peritumoral and perivascular TAM, suppressed tumor angiogenesis and lymphangiogenesis, disorganized extracellular matrices and concomitantly dramatically suppressed metastasis and improved prognosis (102). Here, CSF1R is linked to osteosarcoma.