Our study of IL8 and TNF-α gene expression suggests that this newly established porcine intestinal epithelial cell line could respond similarly to bacterial infection, we also showed that the ZYM-SIEC02 cells increased secretion of IL-8 infection by S. Typhimurium. This is in agreement with previous observations showing increased levels of IL-8 production in the serum of S. Typhimurium infected piglets [49], and is also in accordance with a marked upregulation of IL-8 secretion observed in human intestinal epithelial cells and IPEC-J2 cells following S. Typhimurium infection [2], [50]. Here, CXCL8 is linked to bacterial infectious disease.