In this study, using a lipophilic, NIR-fluorescent cyanine cell membrane dye and a direct labeling method, we investigated the tumor localization and migration of 4T1 tumor specific T-lymphocytes that were first activated in vitro with Bryostatin/Ionomycin (B/I) and grown with Interleukin-2 (IL-2). Here, IL2 is linked to neoplasm.