Finally, we selected the top 3 survival associated genes, GYS1, TGFB1I1 and GABRA3, for validation by qPCR in 6 glioma cell lines (HA and H4, human astrocytes and astrocytoma cell line; N1 and N15, primary culture AII cells; N18, primary culture grade II oligodendroastrocytoma; N14, primary culture grade III oligodendroastrocytoma cell) and additional 49 samples (36 AIIs and 13 AIIIs). This evidence concerns the gene GABRA3 and astrocytoma (excluding glioblastoma).