In addition, a missense mutation at V123M was reported among 1/31 primary melanomas.[29] The B-box1 domain of TRIM protein, MID1, structurally resembles the ring domain of E3 ubiquitin ligases.[30] TRIM16 has a 46% sequence similarity with MID1, can adopt ring domain-like folds and has E3 ligase function in vitro and in vivo [31], indicating that mutation in TRIM16 B-box1 may perturb E3 ligase activity. This evidence concerns the gene TRIM16 and melanoma.