Thus, genetic diagnosis by conventional techniques of a new ADPKD family requires long-range polymerase chain reaction (LR-PCR) of the repeated region of PKD1 followed by nested PCRs (Rossetti et al. 2002), combined with Sanger sequencing of all 46 PKD1 and 15 PKD2 exons. Here, PKD2 is linked to autosomal dominant polycystic kidney disease.