PKD1 and autosomal dominant polycystic kidney disease: Thus, genetic diagnosis by conventional techniques of a new ADPKD family requires long-range polymerase chain reaction (LR-PCR) of the repeated region of PKD1 followed by nested PCRs (Rossetti et al. 2002), combined with Sanger sequencing of all 46 PKD1 and 15 PKD2 exons.