Then, the DNA methylation status of 193 CpG units including 299 CpG sites in the remaining 14 ccRCC-specific CIMP marker genes, i.e. FAM150A, GRM6, ZNF540, ZFP42, ZNF154, RIMS4, PCDHAC1, KHDRBS2, ASCL2, PRAC, WNT3A, TRH, ZNF671 and SLC13A5, was evaluated quantitatively using the MassARRAY system. This evidence concerns the gene TRH and nonpapillary renal cell carcinoma.