In this study, the pharmacokinetics of Dox and PTX was unified through the encapsulation of both drugs into a single cMLV particle, resulting in dual drug-loaded cMLVs which successfully reduced P-gp expression, increased the cellular accumulation of drugs, and enhanced cytotoxicity in cancer cells, including drug-resistant cells, as compared to single drug-loaded cMLVs. This evidence concerns the gene PGP and cancer.