Since the highly functional TILs resulting from PROSTVAC immunotherapy greatly outnumbered the tumor-associated regulatory CD4 Treg population, PROSTVAC immunotherapy considerably increased the ratios of CD4 and CD8 effector T cells to suppressive Treg cells (Teff : Treg ratio) in the tumors (Figure 6B and C). The gene discussed is CD8A; the disease is neoplasm.