Our analyses in melanoma patients supported the elevated pro-apoptotic phenotype of TAA-specific (vs. FluM1-specific) CD4+ T cells based on Annexin-V+ staining in flow cytometry assays, which was clearly enriched within the Type-1 (T-bet+) sub-population of TAA-tetramer+ events across all tumor-associated antigenic specificities evaluated in this study. This evidence concerns the gene ANXA5 and melanoma.