Ao staining showed much lower levels of red fluorescent autophagosome in GANT-61 treated MYCN non-amplified NB cells than those in GANT-61 treated MYCN amplified NB cells (Figure 5D), indicating that MYCN amplification is likely to have a role in GANT-61 induced autophagy in NB cells. Here, MYCN is linked to neuroblastoma.