IHC analysis showed relatively high protein expression of Notch2 in the tumor tissues inoculated with naive U87 cells or U87 cells stably transfected with negative-shRNA (yellow-brown to brown color), but almost no Notch2 protein expression was observed in the tumor tissues inoculated with U87 cells stably transfected with Notch2-shRNA on day 40 after inoculation (Fig. 4A). The gene discussed is NOTCH2; the disease is neoplasm.