↓ AOM-induced promoter demethylation ↓ AOM-induced expression of Sfrp2, Sfrp5 and Wnt5a ↓ RNA PolII binding to Sfrp2 (GEN), Sfrp5 and Wnt5a promoter ↓ AcH3, H3K9me3, H3S10P at Sfrp2, Sfrp5, Wnt5a promoter ↑ nuclear HDAC3 expression. The gene discussed is WNT5A; the disease is infectious otitis media.