Further insights also might come from ChIP assays following treatment with MSC and a transaminase inhibitor, such as aminooxyacetic acid (AOAA; Figure 1); however, such experiments would have to be optimized to account for the time taken to convert MSC to MSP at levels that might inhibit HDAC activity.10,11 We did not specifically examine changes in BMF expression or HDAC promoter occupancy in other cell lines shown (Supplementary Figure 4), or in colon cancer cells other than HCT116 and HT29 (Figure 2a). This evidence concerns the gene HDAC9 and malignant colon neoplasm.