On the other hand, if we were to include the 1+ samples as positive, like Capper et al. did in their first paper [9], we would increase the number of suspected mutations to 101/285 which would clearly be wrong based on the data from the DNA-based methods as well as the previously reported frequencies of BRAF mutations in primary CRC (10 %) as well as in surgically removed metastasis (∼2 %) [31, 32]. Here, BRAF is linked to colorectal carcinoma.