We developed a strategy where NY-ESO-1 specific T cells were first enriched through in vitro stimulation of CD25 depleted PBMC [17] with peptide pulsed dendritic cells in the presence of IL-21, followed by tetramer guided cell sorting to isolate and expand autologous NY-ESO-1-specific CTL from the peripheral blood of patients with sarcoma under clinically compliant manufacturing conditions. Here, IL21 is linked to sarcoma.