Here we demonstrated that after stimulating CD25 depleted T cells from sarcoma patients with autologous dendritic cells pulsed with the NY-ESO-1157-165 peptide and in vitro exposure to IL-21, rare wells containing measurable tetramer positive T cells can be identified and clinical grade cell sorting can be used to enrich and expand these populations to large numbers of highly purified tumor-reactive T cells. The gene discussed is IL21; the disease is sarcoma.