CD4 and infection: To provide further evidence for a critical role of Tcm generated by treatment with immunomodulators during BCG vaccination in resistance to M. tuberculosis infection, we immunized Thy1.2 congenic mice and treated these animals with suplatast tosylate and D4476, isolated CD4+ T cells, and adoptively transferred them into irradiated Thy1.1 congenic animals followed by infection with H37Rv (Fig. 4A).