IRS1 and hydrops fetalis: After 2 h of reoxygenation, western blot analysis revealed that Lin28a overexpression was associated with a significant increase in IGF1R, Nrf-1, Tfam and phosphorylation of IRS-1, Akt, mTOR, p70s6k, AMPK protein levels while a significant decrease in Ace-lysine-PGC-1α protein levels in cardiomyocytes that were exposed to H/R injury in HG/HF incubation (Figure 4).