CASP3 and hydrops fetalis: H/R-aggravated apoptosis in HG/HF incubation, as demonstrated by enhanced expression levels of Cleaved Caspase-3, Caspase-3, Bax protein and Bax/Bcl-2 ratio (Figure 2E–2J), increased TUNEL (red) staining in cardiomyocytes (Figure 2A, 2B) and facilitated cellular apoptosis by flow cytometry analysis (Figure 2C, 2D).