To clarify whether E5 inhibits leukemia cells migration and adhesion to the stromal cells through affecting the intracellular signaling of CXCR4/CXCL12, we therefore conducted western blot analysis for activation of Erk (phospho-Erk), Akt (phosphor-Akt) and p38/MAPK (phospho-p38) in the four kinds of leukemia cell. Here, AKT1 is linked to leukemia.