To understand the progression of brainstem pathology, we analysed RML‐ and ME7‐inoculated MloxP mice at late stages of prion disease (RML, 12 wpi; ME7, 16 wpi); and NFH‐Cre/MloxP mice at intermediate (RML, 12 wpi; ME7, 16 wpi) or late (RML, 35 wpi; ME7, 28 wpi) stages of prion disease (Figure 4). Here, NEFH is linked to prion disease.