Although it is not known whether other metastasis-related miRNAs are suppressed by Hotair, or how Hotair is dramatically upregulated in metastatic breast cancer cells, our study suggests a novel pattern of metastatic cell signaling, in which Hotair represses a tumor suppressor miRNA and thereby maintains high levels of key transcription factors and their target gene products involved in metastasis. This evidence concerns the gene HOTAIR and breast cancer.