Moreover, using a heterologous cell system based on GC cells (rat pituitary tumour cell line) stably expressing the hGHRH-receptor (GC-GHRHR cells) which were transiently transfected with wt-hGH, we investigated the potential role of GPR41 and 43 in butyrate-induced Ca2+ signalling and its possible impact on hGH secretion. This evidence concerns the gene GHRHR and pituitary tumor.