Esposito et al. [43] also reported that changes in life style aimed at reducing body weight and increasing physical activity over 1 year significantly reduced high IL-10 levels in obese women, while Manigrasso et al. [44] reported no significant change in IL-10 levels after an observed reduction in body weight in another obesity study. This evidence concerns the gene IL10 and obesity due to melanocortin 4 receptor deficiency.